Waters XBridge Columns Manuel d'utilisateur

[ Care and Use ManUal ]XBridge™ Columns 1Thank you for choosing a Waters XBridge™ column. The XBridge™ packing materials were designed to provide exce

[ Care and Use ManUal ]XBridge™ Columns 102. Equilibrate with 20 column volumes of initial mobile-phase conditions before making first injection. 3.

[ Care and Use ManUal ]XBridge™ Columns 11Mobile Phase Considerations1. Always maintain at least 5% polar solvent in the mobile phase or gradient (e.

[ Care and Use ManUal ]Waters Corporation 34 Maple Street Milford, MA 01757 U.S.A. T: 1 508 478 2000 F: 1 508 872 1990 www.waters.comXBridge™ Columns

[ Care and Use ManUal ]XBridge™ Columns 2I. GettInG startedEach XBridge™ column comes with a Certificate of Analysis and a Perfor-mance Test Chromatog

[ Care and Use ManUal ]XBridge™ Columns 3Table 1: Empty Column Volumes in mL (multiply by 10 for flush solvent volumes)Column internal diameter (mm)Co

[ Care and Use ManUal ]XBridge™ Columns 4Table 2: Recommended pH and temperature Limits for XBridge™ Columns at Ambient Temperatures Name of Column P

[ Care and Use ManUal ]XBridge™ Columns 5III. sCalInG up/down IsoCratIC methodsThe following formulas will allow scale up or scale down, while maintai

[ Care and Use ManUal ]XBridge™ Columns 6Note: If a column has been run with a mobile phase that contains for mate (e.g., ammonium formate, formic aci

[ Care and Use ManUal ]XBridge™ Columns 7Figure 4: Waters Ferrule in a Parker Style EndfittingThere are two ways to fix the problem:1. Tighten the sc

[ Care and Use ManUal ]XBridge™ Columns 85. Measure the peak width at 4.4% of peak height (5-sigma method): 5-sigma Bandspreading (µL) = Peak Width

[ Care and Use ManUal ]XBridge™ Columns 9b. Impact of Bandspreading Volume on 2.1 mm i.d. Column Performance System with 70 µL bandspreading: 10,000