Waters Oligonucleotide Separation Technology Standard Manuel d'utilisateur télécharger pdf

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MassPREP Oligonucleotide Separation Technology Standard 1

Contents

I. IntroduCtIon

II. reCommended usage

III. PreParatIon ProCedure

IV. storage and stabIlIty

V. orderIng InformatIon

massPreP olIgonuCleotIde seParatIon teChnology standard

I. IntroduCtIon

The pre-packaged MassPREP™ Oligonucleotide Separation

Technology (OST) Standard is designed for verification of HPLC/

UPLC

instrument and column performance for analysis of synthetic

oligonucleotides. Approximately equimolar amounts of 15, 20, 25,

30 and 35 nucleotide (nt) long oligodeoxythymidines are lyophilized

and packaged in 1.5 ml LC vials. These vials are vacuum-sealed in

foil pouches to reduce degradation that can occur by excessive expo-

sure to light and air. Approximately 1 nmole of each oligonucleotide

is present in the vial.

II. reCommended usage

Waters MassPREP OST standard is intended for testing the column and

HPLC/UPLC systems separation performance. For selection of Waters

XBridge™ OST C18, 2.5 µm HPLC or ACQUITY UPLC

OST C18, 1.7 um

UPLC column, see document 720002008EN on waters.com/library. Both

columns and LC system performance can be tested using this dedicated

MassPREP OST standard. The most common problems in oligonucleotide

analysis that can be detected using OST standard are listed in the Table 1.

1 2 3 4

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Page 1 - [ Care and Use ManUal ]

[ Care and Use ManUal ]MassPREP Oligonucleotide Separation Technology Standard 1ContentsI. IntroduCtIonII. reCommended usageIII. PreParatIon Pro

Page 2

[ Care and Use ManUal ]MassPREP Oligonucleotide Separation Technology Standard 2Table 1: System Troubleshooting using MassPREP OST StandardIII. PrePar

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[ Care and Use ManUal ]MassPREP Oligonucleotide Separation Technology Standard 3Figure 1: ACQUITY UPLC analysis of MassPREP OST standard on an ACQUITY

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[ Care and Use ManUal ]Waters Corporation 34 Maple Street Milford, MA 01757 U.S.A. T: 1 508 478 2000 F: 1 508 872 1990 www.waters.comMassPREP Oligonuc

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Waters Oligonucleotide Separation Technology Standard Manuel d'utilisateur

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