Waters SunFire Columns Manuel d'utilisateur

[ Care and Use ManUal ]ContentsI. GETTING STARTED a. Column Installation b. Column Equilibration c. Initial Column Efficiency Determination II

[ Care and Use ManUal ]Waters Corporation 34 Maple Street Milford, MA 01757 U.S.A. T: 1 508 478 2000 F: 1 508 872 1990 www.waters.com© 2010 Waters Co

[ Care and Use ManUal ]2I. GETTING STARTEDEach SunFire column comes with a Performance Test Chromatogram. This Performance Test Chromatogram is specif

[ Care and Use ManUal ]32. Purge the column with the mobile phase until you obtain a stable baseline.3. Verify that retention times and peak areas

[ Care and Use ManUal ]4c. pH RangeThe recommended operating pH range for SunFire columns is 2 to 8. A listing of commonly used buffers and additives

[ Care and Use ManUal ]5III. SCALING UP/DOWN ISOCRATIC METHODSThe following formulas will allow scale up or scale down, while maintaining the same lin

[ Care and Use ManUal ]6b. StorageCompletely seal the column to avoid evaporation and drying out of the bed.Reversed-phase Columns (SunFire C18 and Su

[ Care and Use ManUal ]7The presence of a void in the flow stream reduces column performance. This can occur if a Parker style ferrule is connected to

[ Care and Use ManUal ]8b. Bandspreading MinimizationFigure 6 shows the influence of tubing internal diameter on system bandspreading and peak shape.

[ Care and Use ManUal ]91. Remove column.2. Use acetonitrile as mobile phase A, and acetonitrile with 0.05 mg/mL uracil as mobile phase B (elimin