Waters Oligonucleotide Separation Technology XBridge OST Manuel d'utilisateur

[ method guidelines ]XBridge™ OST C18 columns are based on Waters second generation of hybrid-silica BEH Technology™ particles and can be effectively

2[ method guidelines ]i. p ri nCip l e s oF o l ig onu C l eot i d e sepa r at ions Separations of detritylated synthetic oligonucleotides on an XBr

3[ method guidelines ]Figure 3: Effectiveness of Waters BEH Technology™ Hybrid-Silica C18 Particle Size on Deoxythymidine Ladder SeparationsHPLC syste

4[ method guidelines ]3. To remove particulates the sample may be filtered with a 0.2 μm membrane. Be sure that the selected membrane is compatible an

5[ method guidelines ]As illustrated in Figures 5 through 7, these analyses were performed with the following mobile phases:Mobile Phase A: 0.1 M TE

6[ method guidelines ]Figure 5: Analysis of a Digested 25mer Phosphorothioate Oligonucleotide HPLC system: Waters BioAlliance™ 2796, PDA Detector

7[ method guidelines ]Once the separation has been optimized, one begins preparing for the preparative separation. The steps to successfully scale a s

Waters Corporation 34 Maple Street Milford, MA 01757 U.S.A. T: 1 508 478 2000 F: 1 508 872 1990 www.waters.com[ method guidelines ]Waters, XBridge, BE