[ Care and Use ManUal ]
Waters Corporation
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Milford, MA 01757 U.S.A.
T: 1 508 478 2000
F: 1 508 872 1990
www.waters.com
XBridge
™
Columns 12
g. For HPLC/MS separations of more complex sugar mixtures (e.g.,
polysaccharides), using XBridge Amide columns typical gradi-
ent conditions include (add NH
4
OH modifier to aqueous and
organic mobile phase reservoirs):
i. Gradient going from 75% to 45% ACN with 0.1% NH
4
OH, 35 °C
6. More complex sample mixtures may require the use of gradient
conditions and/or longer column lengths.
8. Typical sample preparation suggestions for samples that contain
sugars/saccharides/carbohydrates:
a. Liquid Samples
i. Dilute with 50:50 ACN/H
2
O
ii. Filter using 0.45 µm or 0.22 µm syringe filter (if necessary)
b. Solid Samples
i. Weigh out sample (~3 g) into 50 mL centrifuge tube
ii. Add 25 mL of 50:50 ACN/H
2
O and homogenize (mechanically)
iii. Centrifuge at 3200 rpm for 30 minutes
iv. Collect supernatant and filter using 0.45 µm or 0.22 µm
syringe filter (if necessary)
c. Depending on sample and/or analyte concentrations, additional
sample dilutions may be necessary.
d. More complex samples and/or lower analyte concentrations
may require additional sample preparation steps and/or proce-
dures such as solid phase extraction (SPE).
e. Consider guard columns for column protection.
©2012 Waters Corporation. Waters, The Science of What’s Possible,
Oasis, SepPak and XBridge are trademarks of Waters Corporation.
Acrodisc is a trademark of Pall Life Sciences. SLIPFREE is a trademark
of Keystone, Inc.
Produced in the U.S.A.
June 2012 720001366EN Rev 7 VW-PDF
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(10 pages)
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