Waters ACQUITY UPLC M-Class Columns Manuel d'utilisateur

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[ CARE AND USE MANUAL ]
INTRODUCTION
Waters
®
ACQUITY UPLC
®
M-Class Columns are manufactured
to exacting specifications and are designed for use at pressures
up to 15000 psi. In order to achieve maximum performance, it is
important to ensure that proper connections are made to minimize
peak tailing and poor efficiency.
Each ACQUITY UPLC M-Class Column is individually tested to
ensure that it passes stringent quality control. Compared to
standard flow chromatography, successful day-to-day performance
of capillary-flow systems can require extra attention to detail.
This document provides several essential recommendations for
the successful use of ACQUITY UPLC M-Class Columns.
ACQUITY UPLC M-Class Columns
ACQUITY UPLC M-Class System.
ACQUITY UPLC M-Class Columns are available in 75, 100, 150, and 300 μm I.D.s.
TABLE OF CONTENTS
I. PREPARING ELUENTS
II. SAMPLE PREPARATION
III. PREPARING AND CONNECTING 75, 100, AND
150 µM I.D. ACQUITY UPLC M-CLASS COLUMNS
IV. PREPARING AND CONNECTING 300 µM I.D.
ACQUITY UPLC M-CLASS COLUMNS
V. CARE WHEN STOPPING FLOW
VI. HOW TO DIAGNOSE AND ADDRESS ABNORMALLY
HIGH BACKPRESSURE AND LEAKS
VII. CHECKING AND CLEANING A FOULED EMITTER
VIII. LONG TERM COLUMN STORAGE
IX. ORDERING INFORMATION
Note: Gloves should be worn for ALL operations
detailed in this document.
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Résumé du contenu

Page 1 - ACQUITY UPLC M-Class Columns

[ CARE AND USE MANUAL ]INTRODUCTIONWaters® ACQUITY UPLC® M-Class Columns are manufactured to exacting specifications and are designed for use at press

Page 2 - [ CARE AND USE MANUAL ]

2[ CARE AND USE MANUAL ]ACQUITY UPLC M-Class Column Care & UseI. PREPARING ELUENTS Do not filter organic solvents. Use MS grade solvents directl

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3[ CARE AND USE MANUAL ]Carefully grab the teflon tubing with one hand and the BARE FUSED SILICA capillary, not the PEEK tubing, with the other hand,

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4[ CARE AND USE MANUAL ]Problem If a clog or a blockage is created after the ACQUITY UPLC M-Class Column (e.g., at emitter) the pressure at the outl

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5[ CARE AND USE MANUAL ]Diagnosis 3 A clogged emitter may still produce an ion signal. The charged emitter may act as an APCI needle and ionize th

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6[ CARE AND USE MANUAL ]1. Remove the clogged emitter and the column from the union on the universal sprayer. 2. Remove the PEEK nuts on either en

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7[ CARE AND USE MANUAL ]Replace the Emitter Place the pin plug at the back side of the union. This will mark the center of the union (right). Thre

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8[ CARE AND USE MANUAL ]What flow rate can I use with each type of emitter? The flow rate of an LC method should be determined by the optimal flow r

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Waters Corporation 34 Maple Street Milford, MA 01757 U.S.A. T: 1 508 478 2000 F: 1 508 872 1990 www.waters.com[ CARE AND USE MANUAL ]Waters, The Scien

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