Waters MassPREP Micro Desalting Column Manuel d'utilisateur télécharger pdf

CONTENTS

I. INTRODUCTION

II. CONNECTING THE MASSPREP MICRO

DESALTING COLUMN WITH THE CHOSEN

LC SYSTEM

III. LC/MS SYSTEM CONFIGURATION TO

MINIMIZE MS SOURCE CONTAMINATION

IV. USING MASSPREP MICRO DESALTING

COLUMN TO MINIMIZE PROTEIN CARRYOVER

BETWEEN INJECTIONS

V. GUIDELINES FOR USE

VI. RECOMMENDED STORAGE

I. INTRODUCTION

Mass spectrometry is a powerful tool for intact protein analysis.

However, the quality of analyses can be compromised by salts in

the sample that interfere with ionization and/or form adducts with

the investigated proteins. It is, therefore, important to remove or

significantly minimize the introduction of extraneous salt ions into

the mass spectrometer. The use of on-line, desalting devices can

efficiently perform this function with minimal sample handling.

Waters MassPREP™ Micro Desalting Columns (2.1 x 5 mm, P/N

186004032) contain a polymer-based, reversed-phase packing

material (20 µm particle size, 1000 Å pore size) that can be used

to effectively desalt protein samples prior to mass spectrometry.

The phenyl phase material contained in these on-line devices

successfully retains proteins allowing the salts to be washed

to waste prior to elution of the desalted protein into the mass

spectrometer. With an optimized LC/MS method, cycle times as low

as 4 minutes are achievable. These on-line devices, when used with

an appropriate LC system injector and injector wash solution, can

be used for the sequential analyses of different samples.

II. CONNECTING THE MASSPREP MICRO DESALTING

COLUMN WITH THE CHOSEN LC SYSTEM

The MassPREP Micro Desalting Column can be used with an

appropriately performing LC system to perform the desalting

functions so long as proper fittings/tubings are used to make

connections. However, optimized performances are achieved

only when the cartridge is connected to a Waters ACQUITY or

nanoACQUITY UPLC system. For the use of the cartridge with

ACQUITY or nanoACQUITY, pre-assembled tubing kits (Intact Mass

Tubing Kit, p/n 205000548) are provided to connect the column to

the UPLC systems and mass spectrometer.

There are two configurations to connect the cartridge into the LC

fluidic path. The inlet of the cartridge can be directly screwed onto

the Valco

UPLC injection valve with no tubing connection.

MassPREP Micro Desalting Column

[ CARE AND USE MANUAL ]

1 2 3

[ CARE AND USE MANUAL ] 1

Résumé du contenu

Page 1 - [ CARE AND USE MANUAL ]

CONTENTS I. INTRODUCTION II. CONNECTING THE MASSPREP MICRO DESALTING COLUMN WITH THE CHOSEN LC SYSTEMIII. LC/MS SYSTEM CONFIGURATION TO

Page 2

This configuration would leave the cartridge operating at room temperature. The other configuration is to screw the cartridge into one end of the incl

Page 3

VI. RECOMMENDED STORAGEFor overnight storage, continuously flush the column with the mobile phase at slow flow (e.g., 50 µL/min for ACQUITY® System).

Commentaires sur ces manuels

Pas de commentaire

Waters MassPREP Micro Desalting Column Manuel d'utilisateur

Naviguer en ligne ou télécharger Manuel d'utilisateur pour Équipement pour l'eau Waters MassPREP Micro Desalting Column. Waters MassPREP Micro Desalting Column User Manual Manuel d'utilisatio

Résumé du contenu

Commentaires sur ces manuels

Produits connexes et manuels pour Équipement pour l'eau Waters MassPREP Micro Desalting Column