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[ CARE AND USE MANUAL ]

HILIC Quality Control Reference Material

CONTENTS

I. INTRODUCTION

II. STORAGE AND STABILITY

III. EXAMPLE OF USING THE HILIC QCRM

IV. ORDERING INFORMATION

I. INTRODUCTION

The Quality Control Reference Material (QCRM) portfolio is a unique

collection of standards and mixtures. These products allow the user to

evaluate and benchmark their chromatography system before analysis

of critical material. The products in the portfolio are all specifically

formulated based on the expertise of Waters scientist.

The retention of polar analytes and metabolites is a chromatographic

challenge for scientists. Polar compounds often elute in the void of most

reversed-phase columns or end up co-eluting with endogenous species

present within various sample matrices in RPLC. Hydrophilic interaction

chromatography (HILIC) is a complementary technique to reversed phase

and normal phase, that can be used to successfully improve the retention

of very polar species.

The HILIC QCRM was designed as a standard to help benchmark this type

of separation. It is a four component mix containing an un-retained void

marker, a polar neutral compound, and two polar basic compounds that

provides a stable chromatographic separation for HILIC columns.

HILIC QCRM Components

Compound Name Property Concentration in Vial (mg/mL)

Acenaphthene V0 marker 0.0190

Thymine Polar neutral 0.0037

Adenine Polar basic 0.0037

Cytosine Polar basic 0.0077

Figure 1. Individual Compound Formulas.

acenaphthene cytosinethymine adenine

1 2 3

[ CARE AND USE MANUAL ] 1

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[ CARE AND USE MANUAL ]HILIC Quality Control Reference MaterialCONTENTSI. INTRODUCTIONII. STORAGE AND STABILITYIII. EXAMPLE OF USING THE HILIC QCRM

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2[ CARE AND USE MANUAL ]HILIC Quality Control Reference MaterialII. STORAGE AND STABILITYThe standard mix comes in a Waters crimp top vial for direct

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Waters Corporation 34 Maple Street Milford, MA 01757 U.S.A. T: 1 508 478 2000 F: 1 508 872 1990 www.waters.com[ CARE AND USE MANUAL ]IV. ORDERING INFO

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Waters HILIC QC Reference Material Manuel d'utilisateur

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