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[ CARE AND USE MANUAL ]

Quantitative Peptide Retention Standard

TABLE OF CONTENTS:

I. INTRODUCTION

II. STORAGE AND STABILITY

III. RECONSTITUTION OF THE PEPTIDE

RETENTION STANDARD

IV. EXAMPLE OF USING THE PEPTIDE

RETENTION STANDARD ON THE

ACQUITY UPLC

V. ORDERING INFORMATION

I. INTRODUCTION

This standard was specifically designed and formulated as a simple peptide

mixture that provides a broad retention window for a variety for a variety of

peptide separation applications. It is an ideal reference material since the

peptides were designed to be stable and to not be prone to modification such

as oxidation.

This standard complements it’s qualitative counterpart, the MassPREP™

Peptide Standard which offers a versatility of various peptides but it not

quantitative. Each peptide in the Quantitative Peptide Retention Standard

is quantitated via amino acid analysis to provide greater accuracy to each

analysis, has been verified by mass identification and is highly pure (more

than 95% pure). Each standard is provided in a Waters TruView™ Maximum

Recovery Vial which simplifies the sample preparation process and allows

for direct injection once solubilized.

The standard consists of four neutral peptides with a single A280

chromophore and varying hydrophobicities and is formulated such that

the peptide components are present in equal abundance. See Table 1 for

specific peptide information.

Table 1: Peptide Mixture Components and Example Data Generated

from Figure 1

Peptide Retention Standard

Peak Area

Peptide MWmono RT (min)* (A

280

) % Area

DGYGK

538.2387

2.6 2446 24

DTVGYGVK 837.4232 10.5 2572 25

DFVGYGVK 883.4440 13 2801 27

DFVGYGVKDFVGVGVK

1684.8825

16.4 2437 24

280 nm chromophore (Y) highlighted

* See Figure 1 for retention time examples.

Applications of the standard can include, but are not limited to: evaluation

of columns and LC systems dedicated to peptide analysis as well as

monitoring performance and recovery across romatographic gradients.

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[ CARE AND USE MANUAL ]Quantitative Peptide Retention StandardTABLE OF CONTENTS: I. INTRODUCTIONII. STORAGE AND STABILITYIII. RECONSTITUTION OF THE

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2[ CARE AND USE MANUAL ]Quantitative Peptide Retention StandardII. STORAGE AND STABILITYLyophilized peptides generally have excellent stabilities, oft

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Waters Corporation 34 Maple Street Milford, MA 01757 U.S.A. T: 1 508 478 2000 F: 1 508 872 1990 www.waters.com[ CARE AND USE MANUAL ]00.060.120510 15

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Waters Quantitative Peptide Retention Standard Manuel d'utilisateur

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