Waters ACQUITY UPLC BEH Columns Manuel d'utilisateur

CONTENTSI. INTRODUCTIONII. GETTING STARTED a. Column Connectors b. Column Installation c. Column Equilibration d. eCord Installation e. Initial

c. Getting Started With ACQUITY UPLC BEH HILIC Columns1. Because ACQUITY UPLC BEH HILIC Columns do not posses a bonded phase, the pH operating range

Injection Solvents1. If possible, injection solvents should be 95% acetonitrile. The polar solvent (i.e., water, methanol, isopropanol) should be min

in the sample are acceptable. 50:50 acetonitrile:water can provide satisfactory results.3. The injection solvent’s influence on peak shape should be

7. If acetone is used in one or more mobile phases, do not use acetone as a sample diluent or needle wash solvent. Refer to injection solvents sectio

II. GETTING STARTEDEach ACQUITY UPLC BEH Column comes with a Certificate of Analysis and Performance Test Chromatogram embedded within the eCord inte

d. eCord InstallationThe eCord button should be attached to the side of the column heater module. The eCord button is magnetized and does not require

II. COLUMN USETo ensure the continued high performance of ACQUITY UPLC BEH Columns, follow these guidelines: a. Sample Preparation 1. Sample impuriti

Table 2. Buffer Recommendations for Using ACQUITY UPLC BEH Columns from pH 2 to 12 Additive/Buffer pKa Buffer range Volatility (±1 pH unit)Used for

III. COLUMN CLEANING, REGENERATING AND STORAGEa. Cleaning and RegenerationChanges in peak shape, peak splitting, shoulders on the peak, shifts in r

b. Storage For periods longer than four days at room temperature, store reversed-phase columns in 100% acetonitrile. For elevated temperature applicat

c. Manufacturing Information d. Column Use InformationThe eCord chip provides the customer with column use data. The top of the screen identifies the

4. Avoid (where possible):100% aqueous mobile phases (if possible).HPLC-grade bottled water.“Topping off” or adding “new” mobile phase to “old”