Waters Amino Acid Analysis Liquid Chromatography Column Manuel d'utilisateur télécharger pdf

[ Care and Use ManUal ]

I. IntroductIon

This manual provides the user with information necessary to obtain

the maximum efficiency and life from the Waters Amino Acid Analysis

Column. This column has been specifically designed for the separation

and analysis of free amino acids. The column is a precision device.

Waters Corporation uses very exacting manufacturing procedures

to achieve a small average particle size, narrow range of particle

size distribution and stable particle structure in the column. These

procedures create the high efficiency column-to-column reproducibility

and long separation life built into each amino acid column from Waters.

However, care must be taken to maintain packing density and protect

it from contamination by foreign material. The following information

will aid you in obtaining the highest performance and longest life from

your column.

II. Background InformatIon

Although there are some hydrophobic interactions between the column

and certain amino acids, the primary mode of separation is that

of ion-exchange. The cation-exchange resin utilized has a large

number of negatively charged (sulfonate) functional groups which

are covalently bound to it. These negatively charged groups, in turn,

readily associate with positively charged cations when they are

present in solution. Before the amino acid sample is injected onto the

column, the resin is equilibrated at a low pH with buffer containing

a high concentration of sodium (Na+) ions. This places the column

in the sodium form. As amino acids enter the column in this acidic

buffer, they are strongly positively charged and readily exchange with

the loosely bound sodium ions.

As the buffer gradually changes to a higher pH during the gradient run,

each amino acid becomes less positively charged reflecting its pK value

and differentially elutes accordingly.

contents

I. INTRODUCTION

II. BACKGROUND INFORMATION

a. Elution System 1

b. Elution System 2

c. General Preparation Guide for One Liter of Eluent

d. Use of Non-aqueous Solvents

e. Backpressure

f. Sample Considerations

g. SEP-PAK

Cartridge Clean Up for Amino Acid Samples

h. Preparation Scheme

III. COLUMN CARE & USE CONSIDERATIONS

a. Column Care Considerations

b. Column Storage

c. Column Regeneration

d. Troubleshooting

e. Regeneration of Protein and Lipid Contaminated Column

f. Regeneration of Heavy Metal Contaminated Column

g. Available Service and Applications Information

h. Chromatographic Troubleshooting

i. Typical Separation

IV. COLUMN PERFORMANCE CHECKS

V. COLUMN END FITTINGS

a. End Fitting Removal Procedure

b. Column Hookup

c. Storage

APPENDIX A. AMINO ACID ANALYSIS COLUMN SPECIFICATIONS

amIno acId analysIs lIquId chromatography column

1 2 3 4 5 6 7 8 9

[ Care and Use ManUal ] 1

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Page 1 - [ Care and Use ManUal ]

[ Care and Use ManUal ]I. IntroductIonThis manual provides the user with information necessary to obtain the maximum efficiency and life from the Wate

Page 2

[ Care and Use ManUal ]2Figure 1: Ion Exchange Mechanism CAUTION: Introduction of improperly prepared or contaminated buffers or samples onto the resi

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[ Care and Use ManUal ]3c. General Preparation Guide for One Liter of Eluent• Dissolvesaltsinapproximately900mlofpurifiedwater.• AdjustpHw

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[ Care and Use ManUal ]4III. column care & use consIderatIonsa. Column Care Considerations• ALWAYSfiltersamplestopreventexcessivepressureb

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[ Care and Use ManUal ]5f. Regeneration of Heavy Metal Contaminated Column• Oftenmetalscanberemovedbychelationunderbasicandacidiccondition

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[ Care and Use ManUal ]6Figure 3: Amino Acid Analysis – Special Amino Acids Variations in results as compared to the expected chromatogram can usual

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[ Care and Use ManUal ]7All peaks should be sharp and symmetrical. Backpressure should be less than 2,000 psi. If you suspect that the quality of you

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[ Care and Use ManUal ]8b. Column Hookup1. Direction of flow is indicated on the column.2. Connect one adaptor to the inlet and one adaptor to the out

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Waters Amino Acid Analysis Liquid Chromatography Column Manuel d'utilisateur

Naviguer en ligne ou télécharger Manuel d'utilisateur pour Équipement pour l'eau Waters Amino Acid Analysis Liquid Chromatography Column. Waters Amino Acid Analysis Liquid Chromatography Column User Manual Manuel d'utilisatio

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