Waters MassPREP Digestion Standard Mixtures Manuel d'utilisateur télécharger pdf

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MassPREP Digestion Standard Mixtures 1

MassPREP DigEstion stanDaRD MixtuREs

intRoDuction

Two MassPREP protein digestion standard mixtures (MPDS Mix 1 and

MPDS Mix 2) were prepared by individually digesting Yeast Alcohol

Dehydrogenase (ADH, SwissProt P00330), Rabbit Glycogen Phos-

phorylase b (GPB, SwissProt P00489), Bovine Serum Albumin (BSA,

SwissProt P02769), and Yeast Enolase I (ENO, SwissProt P00924)

with sequencing grade trypsin, and producing mixtures in the

indicated molar ratios. The MPDS mixtures are purified, and do not

contain undigested protein, trypsin, or very hydrophilic components

such as salts.

Vial contEnts

stoRagE anD stability

The lyophilized powder is stable at room temperature until the

expiration date printed on the label or two years from date of

purchase. Once reconstituted in 0.1% formic acid, the solution is

stable for one week when stored at 4 °C. Stability can be extended

by freezing samples at -20 °C.

Note: The contamination of sample with endo- or exopeptidases may

cause rapid sample degradation.

Reconstitution of MPDS mixtures for analysis with the Waters Protein

ExP REssion systEM using nanoscalE

(75 µM scalE) HPlc

Each MPDS mixture is prepared by resuspension of the lyophilized

powder in 1.0 mL of 0.1% formic acid. Mix capped vial by vortex

to insure all material is resuspended. Refer to the Waters Protein

Expression System Operator’s Guide for detailed system level test

procedures employing the two MPDS mixtures.

Reconstitution of MPDS mixtures for analysis with the Waters Protein

ExPREssion systEM using MicRoscalE

(300 µM scalE) HPlc

Each MPDS mixture is prepared by resuspension of the lyophilized

powder in 100 µL of 0.1% formic acid. Mix capped vial by vortex

to insure all material is resuspended. Refer to the Waters Protein

Expression System Operator’s Guide for detailed system level test

procedures employing the two MPDS mixtures.

MassP REP DigEstion stanDa RD oRDERing

infoRMation

Protein MPDS Mix 1 [Molar ratio,

Amount (pmol)]

MPDS Mix 2 [Molar ratio,

Amount (pmol)]

ADH 1.0, 50 pmol 1.0, 50 pmol

GPB 1.0, 50 pmol 0.5, 25 pmol

ENO 1.0, 50 pmol 2.0, 100 pmol

BSA 1.0, 50 pmol 8.0, 400 pmol

Part Number Product Description Qty per

Pack

186002325 MassPREP Digestion Standard, Enolase 1

186002326 MassPREP Digestion Standard, Phosphorylase b 1

186002327 MassPREP Digestion Standard, Bovine

Hemoglobin

186002865 MassPREP Digestion Standard, Mix 1 1

1 2

[ Care and Use ManUal ] 1

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[ Care and Use ManUal ]MassPREP Digestion Standard Mixtures 1MassPREP DigEstion stanDaRD MixtuREsintRoDuctionTwo MassPREP protein digestion standard m

Page 2

[ Care and Use ManUal ]Waters Corporation 34 Maple Street Milford, MA 01757 U.S.A. T: 1 508 478 2000 F: 1 508 872 1990 www.waters.comMassPREP Digestio

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Waters MassPREP Digestion Standard Mixtures Manuel d'utilisateur

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