Waters XSelect HSS XP 2.5 µm Columns Manuel d'utilisateur

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XSelect HSS XP 2.5 µm Columns 1
[ CARE AND USE MANUAL ]
CONTENTS
I. INTRODUCTION
II. GETTING STARTED
a. Column Connection
b. Column Installation
c. Minimizing Band Spread Volume
d. Measuring Band Spread Volume
e. Measuring System Dwell Volume
f. Column Equilibration
g. Solvent Conversion for Normal-Phase Use
h. eCord Installation
i. Initial Column Efficiency Determination
j. VanGuard Pre-Columns
III. COLUMN USE
a. Sample Preparation
b. pH Range
c. Solvents
d. Pressure
e. Temperature
IV. COLUMN CLEANING, REGENERATION AND STORAGE
a. Cleaning and Regeneration
b. Storage after Reversed-Phase Use
c. Storage after HILIC Use
V. eCORD INTELLIGENT CHIP TECHNOLOGY
a. Introduction
b. Installation
c. Manufacturing
d. Column Use
VI. ADDITIONAL INFORMATION
a. Tips for Maximizing XSelect HSS XP 2.5 µm Column Lifetime
b. Troubleshooting Questions
c. Recommended Flow Rates and Anticipated Backpressures for
Reversed-Phase XSelect HSS XP 2.5 µm Columns
XSELECT HSS XP 2.5 µm COLUMNS
I. INTRODUCTION
Thank you for choosing a Waters XSelect
High Strength Silica [HSS]
eXtended Performance [XP] 2.5 µm Column. The manufacture of
XSelect HSS XP 2.5 µm Columns begins with ultrapure reagents and
are manufactured in a cGMP, ISO 9001 certified facility to control the
chemical composition and purity of the final product. Well-controlled
manufacturing processes result in industry-leading batch-to-batch
reproducibility. Every column is individually tested. A Performance
Chromatogram and Certificate of Batch Analysis are provided on the
eCord
Intelligent Chip.
XSelect HSS XP 2.5 µm Columns are based on the same base
particle technology and bonded phase chemistry as 1.8 µm ACQUITY
UPLC
®
HSS Columns as well as XSelect HSS 3.5 and 5 µm HPLC
Columns, thus enabling seamless transferability between HPLC,
UHPLC and UPLC
®
platforms.
XSelect HSS XP 2.5 µm Columns will exhibit maximum
chromatographic performance when used on a member of the
ACQUITY UPLC System family.
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Résumé du contenu

Page 1 - XSELECT HSS XP 2.5 µm COLUMNS

XSelect HSS XP 2.5 µm Columns 1[ CARE AND USE MANUAL ]CONTENTSI. INTRODUCTIONII. GETTING STARTED a. Column Connection b. Column Installation c

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XSelect HSS XP 2.5 µm Columns 10[ CARE AND USE MANUAL ]c. Recommended Flow Rates and Anticipated Backpressures for Reversed-Phase XSelect HSS XP 2.5 µ

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Waters Corporation 34 Maple Street Milford, MA 01757 U.S.A. T: 1 508 478 2000 F: 1 508 872 1990 www.waters.comXSelect HSS XP 2.5 µm Columns 11[ CARE A

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XSelect HSS XP 2.5 µm Columns 2[ CARE AND USE MANUAL ]II. GETTING STARTEDEach XSelect HSS XP 2.5 µm Column comes with a Certificate of Analysis and a

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XSelect HSS XP 2.5 µm Columns 3[ CARE AND USE MANUAL ]System Volume4.4 %h5Figure 2: Determination of system band spread volume using 5-Sigma Method.Ta

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XSelect HSS XP 2.5 µm Columns 4[ CARE AND USE MANUAL ]Table 3: Column Volumes (mL)Column Length (mm)Internal Diameter2.1 mm 3.0 mm 4.6 mm30 0.10 0.21

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XSelect HSS XP 2.5 µm Columns 5[ CARE AND USE MANUAL ]Note: VanGuard Pre-Columns are shipped with a collet and ferrule that are NOT pre-swaged. This e

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XSelect HSS XP 2.5 µm Columns 6[ CARE AND USE MANUAL ]Table 5: Buffer Recommendations for XSelect HSS XP 2.5 µm ColumnsAdditive or BufferpKaBuffer Ran

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XSelect HSS XP 2.5 µm Columns 7[ CARE AND USE MANUAL ]b. Storage after Reversed-Phase UseFor periods longer than four days, store the XP 2.5 µm Column

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XSelect HSS XP 2.5 µm Columns 8[ CARE AND USE MANUAL ]c. ManufacturingThe eCord provides the user with the Batch Certificate of Analysis and Performan

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XSelect HSS XP 2.5 µm Columns 9[ CARE AND USE MANUAL ]3. Always remember to:  Use an in-line filter unit (Part No. 205000343) or a VanGuard Pre

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